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Genome organization and evolution of rice (Oryza sativa L.).


The organization and evolution of chromosome 8 and the nucleotide-binding site-leucine-rich repeat (NBS-LRR)- encoding gene family in the genome of rice, Oryza sativa L., were determined in this study. Toward these goals, three large-insert genomic DNA libraries of maize inbred line B73 were generated in pBeloBAC11, pECBAC1, and pCLD04541 vectors using BamHI, EcoRI and HindIII, respectively. The three libraries collectively contain 251, 137 clones, and have average insert sizes of 110, 140 and 115 kilobase pairs, respectively, insert-empty clones of & lt;5% and chloroplast DNA clones of <2%, thus providing 12.3 haploid genome equivalents. Cloned maize DNAs were stable in the vectors, and the clones are highly amenable to genome research of maize and related species.